Coding

Part:BBa_K4350820:Design

Designed by: Ryan Riopel   Group: iGEM22_UBC-Okanagan   (2022-10-09)


N. nambi Fungal Luciferase V49A d33


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is codon-optimized for expression in the model cyanobacterial organism, Synechocystis sp. PCC 6803. Additionally, illegal restriction sites have been removed for compatibility with the MoClo golden gate cloning assembly standard.


Source

The sequence for the enzyme was retrieved from https://www.pnas.org/doi/10.1073/pnas.1803615115. The V49A mutation and 33 amino acid deletion were incorporated into the sequence by UBCO iGEM.

References